Wednesday, February 26, 2020

Conceptualizing internationalization Strategy Case Study

Conceptualizing internationalization Strategy - Case Study Example cate that businesses internationalize, and are better placed to serve customers in countries oversees, and also gain novel ideas on products, services and business methodologies. This piece shall assess the case of Chabros International Group: A World of Wood as an international group and the challenges it has had in the course of carrying out business. According to Farah (2010), Chabros Group went through a downfall that saw a drop in its sales. With the help of the top management, there was need to revive the company immediately to avoid its collapse. Since the company had experienced losses in the countries that it had already ventured into, it would be important if the company tries to restart in another country, most preferably Morocco (Farah, 2010). This is for the reason that, in the countries that it had already operated the company was slowly losing its pride and fame as the leading producer. In an event that another company comes in to replace what it had already started, chance are that, the new company would make more sales and grab a huge percentage of the clients that Chabros had already won (Farah, 2010). This would be attributed to the fact that clients would have lost faith in Chabros and its consistent supply of products, and will probably try their luck in the new producing company. Morocco would be the best destination for Chabros Company to restart its operations. In the work compiled by Farah (2010), Morocco would be the best destination for Chabros to restart as it has minimal chances of facing local competition as there are few sellers of veneer, and there is a big market served by imports. The country is also not going through a recession, and its currency, Dirham, is linked to the Euro; thus, a reduction of Chabros exchange risks (Farah, 2010). With specialized technical know-how, capacity to consistently supply the market with quality products, well conversant with EU’s production measures and Morocco having a positive perception of

Monday, February 10, 2020

DNA Sequencing Essay Example | Topics and Well Written Essays - 4000 words

DNA Sequencing - Essay Example The Sanger methods are able to sequence best from 30-350 nucleotides and therefore genomic sequencing strategies have been developed to sequence longer DNA of interest such as the gene of interest in this plant. In the shotgun sequencing strategies, DNA often of large size is shredded into smaller fragments that can then be sequenced individually. Shredding of the DNA is done by restriction enzymes or mechanically by shearing the DNA. The sequences of these fragments are then reassembled into their original order based on overlaps. Alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise duri ng assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in the assembly of â€Å"gaps†. Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. Pairwise-end sequencing is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun.... Usually alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise during assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in assembly of â€Å"gaps†. . Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. These steps are repeated over and over until the complete sequence of the insert is elucidated. Pairwise-end sequencing (double-barrel shortgun ) is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun. It reduces â€Å"gaps† thereby minimizing assembly errors which are common in whole-gun sequencing. However it poses a huge computational challenge during assembly. DNA is shredded into 150mb fragments and inserted into BACs in hierarchical shortgun sequencing strategy. Inserts are mapped into a physical map and organized by known location â€Å"Golden Tiling Path†. Inserts are fragmented further and cloned into plasmid where they are again recovered and sequenced according to â€Å"the Golden Tiling Path†. This strategy is applied for long pieces of DNA such as whole genome or chromosome and in